Product Description:
Background: Human Lin28, encoded by the LIN28 gene, is a cytoplasmic mRNA-binding protein with a cold shock domain and two CHCC-type zinc fingers. It has been shown to enhance translation of IGF-2 mRNA and to block processing of let-7 microRNA. Lin28 is a marker for undifferentiated embryonic stem cells, and Lin28 expression has been used to enhance the generation of induced pluripotent stem (iPS) cells.
Reacts with |
Human, Mouse* |
Tested Applications |
WB, ELISA, Dot |
Host |
Mouse |
Clone |
PR-4C9 |
Isotype |
IgG1, κ |
*Other species not tested |
Size: 100 µg
Immunogen: Synthetic peptide derived from human Lin28 (between AA 100 and AA 150) conjugated to ovalbumin carrier protein.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS
Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC.
Avoid multiple freeze-thaws.
Recommended Uses:
Western Blot 1:1000
ELISA (detect) 1:1000
Dot blot (detect) 1:500
Note: Optimal concentrations should be empirically determined.
Target Alternative Names: Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein
Target Size: 209 AA; 22.7 kDa
Target Database Links:
RefSeq Protein NP_078950
RefSeq Gene NM_024674
UniProt Q9H9Z2
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