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Media Formulations

Mesenchymal Stem Cell Medium
500mlAdipose derived MSC Medium
450 mlα-MEM
50 mlFetal Calf Serum
1.25 mlL-Glutamine (0.5 mM)
5 mlPenicillin/Streptomycin
Plating Medium
500mlPlating Medium
450 mlMEM (1 mM pyruvate + 0.6% glucose)
with 5 ml Pyruvate (1 mM)
with 2.7 g D-Glucose (0.6% w/v)
50 mlHorse Serum
5 mlPenicillin/Streptomycin
Recommended Sources
ComponentSource
Sodium Pyruvate (100mM)Sigam
#S8636
Horse SerumGibco
#16050-122
L-Glutamine (200mM)Gibco
#25030-081
a-MEMLonza
#12-169F
MEMGibco
#31095-029
Neurobasal MediumGibco
#21103049
B27Gibco
#17504-044
Glutamic AcidSigma
#G1251
D-GlucoseSigma
#G7021
Penicillin/StreptomycinGibco
#15140-122
FungizoneGibco
#15290-018
Fetal Bovine Serum (for hippocampal astrocytes)EuroClone
#ECS0180L
Fetal Bovine Serum (for MSCs)Thermo/Hyclone
#SH30070.03