Nanog is a transcription factor that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. The Nanog protein contains a conserved DNA-binding homeobox domain (AA 98-154). When transfected together with other key pluripotent factors such as OCT4 (Pou5F1) and SOX2, Nanog has been used to enhance the reprogramming of somatic cells into induced pluripotent stem (iPS) cells.
Reacts with: Human*
Tested Applications: WB, ELISA
Isotype: IgG2a, κ
Size: 100 µg
Immunogen: Recombinant full-length human Nanog expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS; Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC. Avoid multiple freeze-thaws.
Western Blot 1:1000-1:10000
ELISA (detect) 1:1000-1:10000
Note: Optimal concentrations should be empirically determined.
*Other species not tested
Target Alternative Names: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
Target Size: 305 residues; 34.6 kDa
Target Database Links:
RefSeq Protein NP_079141.1
RefSeq Gene NM_024865.1