Nanog Recombinant Protein

Description:

Nanog is a transcription factor that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. The Nanog protein contains a conserved DNA-binding homeobox domain (AA 98-154). When transfected together with other key pluripotent factors such as OCT4 (Pou5F1) and SOX2, Nanog has been used to enhance the reprogramming of somatic cells into induced pluripotent stem (iPS) cells.

Source: Human
Tested Applications: SDS-PAGE, WB, ELISA, Dot
Sequence: Full length
Epitope tag(s): none

Size: 20 µg

Description: Recombinant full-length human Nanog protein

Expression: Protein was expressed in E. coli and refolded to soluble form.

Formulation:
110 µg/ml (determined by Bradford assay) in hs-PBS (1.5 mM KH2PO4, 8.1 mM Na2HPO4, 500 mM NaCl, 2.7 mM KCl, pH 7.4) + 5% glycerol
Preservative: none

Purity: >90% by SDS-PAGE

Storage: Store at -80 °C.
Avoid multiple freeze-thaws.

Alternative Names: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

Size: 305 residues; 34.6 kDa

Database Links:
RefSeq Protein NP_079141.1
RefSeq Gene NM_024865.1
UniProt Q9H9S0

Warranty: Each Lot has been verified to meet quality specifications in accordance with testing guidelines. We will replace the product if it does not conform to specifications. Product guaranteed for 6 months from date of purchase if stored as directed. This product must not be resold without written permission from Primorigen Biosciences.

S2099-20UG

S2099