Nkx2.2 is a transcription factor belonging to NK-2 homeobox family. It is expressed in the ventral regions of the developing vertebrate central nervous system (CNS) and is therefore thought to play a role in the morphogenesis of the CNS. In addition, gene knockout experiments in mice have identified Nkx2.2 as a key regulator of insulin-producing beta-cell development in the pancreas. Nkx2.2 is used as a marker for pancreatic islet cell lineages when inducing differentiation of human stem cells.
Reacts with: Human*
Tested Applications: WB, ELISA
Isotype: IgG2a, κ
Size: 100 µg
Recombinant human Nkx2.2 fragment (AA 1-127) expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS
Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC.
Avoid multiple freeze-thaws.
Western Blot 1:1000-1:5000
ELISA (detect) 1:1000
Note: Optimal concentrations should be empirically determined.
Target Alternative Names:
Homeobox protein Nkx-2.2; Homeobox protein NK-2 homolog B; NK2 homeobox 2
Target Size: 273 AA; 30.1 kDa
Target Database Links:
RefSeq Protein NP_002500.1
RefSeq mRNA NM_002509.2
Each Lot has been verified to meet quality specifications in accordance with testing guidelines. We will replace the product if it does not conform to specifications. Product guaranteed for 6 months from date of purchase if stored as directed. This product must not be resold without written permission from Primorigen Biosciences.