POU5F1, commonly known as Octamer-binding protein 4 (Oct4), is a POU transcription factor family member that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. For this reason, POU5F1 expression is frequently used as a marker for undifferentiated stem cells. It contains a POU-specific domain and a homeobox DNA-binding domain. When transfected together with other key pluripotent transcription factors, POU5F1 has been used to reprogram somatic cells into induced pluripotent stem (iPS) cells.
Reacts with: Human*
Tested Applications: WB, ELISA, Dot
Size: 100 µg
Immunogen: Recombinant human POU5F1 (Oct4) fragment (AA 1-212) expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS
Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC.
Avoid multiple freeze-thaws.
Western Blot 1:1000-1:5000
ELISA (detect) 1:1000-1:5000
Dot blot (detect) 1:1000
Note: Optimal concentrations should be empirically determined.
Target Alternative Names: POU domain, class 5, transcription factor 1; Octamer-binding protein 4; Oct-4; Oct-3; Oct-3/4; OTF-3