SOX2 is a member of the large family of sex determining region Y-related HMG-box (SOX) transcription factors. SOX2 is expressed in embryonic stem cell lines, and decreased levels of SOX2 interfere with self renewal and pluripotency. SOX2 has been identified as one of the key regulators for induced pluripotent stem (iPS) cells. The DNA binding domain (AA 41-109) shows significant homology to other SOX family members, while the N-terminal (AA 1-38) and C-terminal (AA 194-317) regions are unique. The Sox2 gene itself contains no introns but lies within an intron of the SOX2 overlapping transcript (SOX2OT) gene.
Reacts with: Human*
Tested Applications: WB, ELISA
Isotype: IgG1, κ
Size: 100 µg
Immunogen: Recombinant full-length human SOX2 expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS; Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC. Avoid multiple freeze-thaws.
Western Blot 1:1000
ELISA (detect) 1:1000
Dot blot (detect) 1:1000
Note: Optimal concentrations should be empirically determined.
*Other species not tested
Target Alternative Names: Sex-determining region Y-box 2, SRY-box 2, ANOP3, MCOPS3, MGC2413
Target Size: 317 AA; 34.3 kDa
Target Database Links:
RefSeq Protein NP_003097.1
RefSeq Gene NM_003106.2